Phosphopeptide elution times in reversed-phase liquid chromatography
نویسندگان
چکیده
منابع مشابه
High speed gradient elution reversed-phase liquid chromatography.
A major disadvantage of gradient elution in terms of speed results from the need to adequately re-equilibrate the column. This work distinguishes two states of re-equilibration: (1) run-to-run repeatability and (2) full equilibration. We find that excellent repeatability (+/-0.002 min in retention time) is achieved with at most 2 column volumes of re-equilibration whereas full equilibration can...
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The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2007
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2007.09.032